Metabolic engineering of microbial competitive advantage for industrial fermentation processes.
Science
; 353(6299): 583-6, 2016 Aug 05.
Article
em En
| MEDLINE
| ID: mdl-27493184
ABSTRACT
Microbial contamination is an obstacle to widespread production of advanced biofuels and chemicals. Current practices such as process sterilization or antibiotic dosage carry excess costs or encourage the development of antibiotic resistance. We engineered Escherichia coli to assimilate melamine, a xenobiotic compound containing nitrogen. After adaptive laboratory evolution to improve pathway efficiency, the engineered strain rapidly outcompeted a control strain when melamine was supplied as the nitrogen source. We additionally engineered the yeasts Saccharomyces cerevisiae and Yarrowia lipolytica to assimilate nitrogen from cyanamide and phosphorus from potassium phosphite, and they outcompeted contaminating strains in several low-cost feedstocks. Supplying essential growth nutrients through xenobiotic or ecologically rare chemicals provides microbial competitive advantage with minimal external risks, given that engineered biocatalysts only have improved fitness within the customized fermentation environment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triazinas
/
Microbiologia Industrial
/
Escherichia coli
/
Biocatálise
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Biocombustíveis
/
Fermentação
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Engenharia Metabólica
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Nitrogênio
Idioma:
En
Revista:
Science
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos