Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.

Gerner, Lisa; Munack, Steffi; Temmerman, Koen; Lawrence-Dörner, Ann-Marie; Besir, Hüseyin; Wilmanns, Matthias; Jensen, Jan Kristian; Thiede, Bernd; Mills, Ian G; Morth, Jens Preben

Gerner, Lisa; Munack, Steffi; Temmerman, Koen; Lawrence-Dörner, Ann-Marie; Besir, Hüseyin; Wilmanns, Matthias; Jensen, Jan Kristian; Thiede, Bernd; Mills, Ian G; Morth, Jens Preben.

Afiliação

Gerner L; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway.
Munack S; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway.
Temmerman K; European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22603 Hamburg, Germany; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Lawrence-Dörner AM; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany; Institute of Biochemistry, WWU Münster, Wilhelm-Klemm-Straße 2, 48149 Münster, Germany.
Besir H; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Wilmanns M; European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22603 Hamburg, Germany.
Jensen JK; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
Thiede B; Department of Biosciences, University of Oslo, Norway; Biotechnology Centre of Oslo, University of Oslo, Norway.
Mills IG; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway; Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospitals, Norway; Movember/Prostate Cancer UK Centre of Excel
Morth JP; Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital, Norway.

Data Brief ; 8: 733-40, 2016 Sep.

Article em En | MEDLINE | ID: mdl-27508226

Palavras-chave

AMPK, adenosine monophosphate-regulated kinase; CaM, calmodulin; CaMK, calcium/calmodulin-dependent kinase; CaMKK2; CaMKK2, calcium/calmodulin-dependent kinase kinase 2; CaMKK2:CaM, CaMKK2-CaM complex; Calmodulin; E. coli, Escherichia coli; Fermentation; IPTG, ß-D-1-thiogalactopyranoside; LB, Luria broth; LDS-PAGE, lithium dodecyl sulphatepolyacrylamide gel electrophoresis; LIC, ligation-independent cloning; OD, optical density; PMSF, phenylmethylsulfonyl fluoride; SEC, size-exclusion chromatography; TEV, tobacco etch virus; aa, amino acid

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Data Brief Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Noruega

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google