Minicircle DNA purification using a CIM® DEAE-1 monolithic support.
J Sep Sci
; 39(18): 3544-9, 2016 Sep.
Article
em En
| MEDLINE
| ID: mdl-27600622
ABSTRACT
Minicircle DNA is a new biotechnological product with beneficial therapeutic perspectives for gene therapy because it is constituted only by the eukaryotic transcription unit. These features improve minicircle DNA safety and increase its therapeutic effect. However, being a recently developed product, there is a need to establish efficient purification methodologies, enabling the recovery of the supercoiled minicircle DNA isoform. Thus, this work describes the minicircle DNA purification using an anion exchange monolithic support. The results show that with this column it is possible to achieve a good selectivity, which allows the isolation of the supercoiled minicircle DNA isoform from impurities. Overall, this study shows a promising approach to obtain the minicircle DNA sample with adequate quality for future therapeutic applications.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Super-Helicoidal
/
Cromatografia por Troca Iônica
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Portugal