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The Ultrastructural Signature of Human Embryonic Stem Cells.
Underwood, Jean M; Becker, Klaus A; Stein, Gary S; Nickerson, Jeffrey A.
Afiliação
  • Underwood JM; Department of Cell and Developmental Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655.
  • Becker KA; Department of Cell and Developmental Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655.
  • Stein GS; Department of Cell and Developmental Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655.
  • Nickerson JA; Department of Biochemistry and Vermont Cancer Center for Basic and Translational Research, University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, Vermont 05405.
J Cell Biochem ; 118(4): 764-774, 2017 04.
Article em En | MEDLINE | ID: mdl-27632380
The epigenetics and molecular biology of human embryonic stem cells (hES cells) have received much more attention than their architecture. We present a more complete look at hES cells by electron microscopy, with a special emphasis on the architecture of the nucleus. We propose that there is an ultrastructural signature of pluripotent human cells. hES cell nuclei lack heterochromatin, including the peripheral heterochromatin, that is common in most somatic cell types. The absence of peripheral heterochromatin may be related to the absence of lamins A and C, proteins important for linking chromatin to the nuclear lamina and envelope. Lamins A and C expression and the development of peripheral heterochromatin were early steps in the development of embryoid bodies. While hES cell nuclei had abundant nuclear pores, they also had an abundance of nuclear pores in the cytoplasm in the form of annulate lamellae. These were not a residue of annulate lamellae from germ cells or the early embryos from which hES cells were derived. Subnuclear structures including nucleoli, interchromatin granule clusters, and Cajal bodies were observed in the nuclear interior. The architectural organization of human ES cell nuclei has important implications for cell structure-gene expression relationships and for the maintenance of pluripotency. J. Cell. Biochem. 118: 764-774, 2017. © 2016 Wiley Periodicals, Inc.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Embrionárias Humanas Limite: Humans Idioma: En Revista: J Cell Biochem Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Embrionárias Humanas Limite: Humans Idioma: En Revista: J Cell Biochem Ano de publicação: 2017 Tipo de documento: Article