Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue.
Biomed Opt Express
; 7(10): 4021-4033, 2016 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-27867712
We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 µm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.
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01-internacional
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MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
Ano de publicação:
2016
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Article