Your browser doesn't support javascript.
loading
Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue.
Nylk, Jonathan; McCluskey, Kaley; Aggarwal, Sanya; Tello, Javier A; Dholakia, Kishan.
Afiliação
  • Nylk J; SUPA, School of Physics and Astronomy, University of St. Andrews, St. Andrews, KY16 9SS, UK.
  • McCluskey K; SUPA, School of Physics and Astronomy, University of St. Andrews, St. Andrews, KY16 9SS, UK.
  • Aggarwal S; School of Medicine, University of St. Andrews, St. Andrews, KY16 9TF, UK.
  • Tello JA; School of Medicine, University of St. Andrews, St. Andrews, KY16 9TF, UK.
  • Dholakia K; SUPA, School of Physics and Astronomy, University of St. Andrews, St. Andrews, KY16 9SS, UK.
Biomed Opt Express ; 7(10): 4021-4033, 2016 Oct 01.
Article em En | MEDLINE | ID: mdl-27867712
We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 µm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biomed Opt Express Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biomed Opt Express Ano de publicação: 2016 Tipo de documento: Article