Halodesulfovibrio spirochaetisodalis gen. nov. sp. nov. and reclassification of four Desulfovibrio spp.
Int J Syst Evol Microbiol
; 67(1): 87-93, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27902290
ABSTRACT
An antibiotic-producing, obligate anaerobic, Gram-stain-negative, catalase- and oxidase-negative strain (JC271T) was isolated from a marine habitat and identified, based on 16S rRNA gene sequence analysis, as a novel member of the family Desulfovibrionaceae. The closest phylogenetic relatives of strain JC271T were found to be Desulfovibrio marinisediminis C/L2T (99.2â%), Desulfovibrio acrylicus W218T (98.7â%), Desulfovibrio desulfuricans subsp. aestuarii (98.6â%), Desulfovibrio oceani subsp. oceani (98.0â%), Desulfovibrio oceani subsp. galatae (98.0â%) and other members of the genus Desulfovibrio (≤91.9â%). To resolve its full taxonomic position, the genomic sequence of strain JC271T was compared to available genomes of the most closely related phylogenetic members. Average Nucleotide Identity scores and DNA-DNA hybridization values confirmed that strain JC271T represents a novel genomic species. Iso-C17 0, iso-C17ââ1ω9c, and iso-C15ââ0 were found to be the major (comprising >10â% of the total present) fatty acids of strain JC271T. Phosphatidylglycerol, phosphatidylethanolamine and unidentified lipids (L1-8) were the polar lipids identified. The G+C content of strain JC271T was 46.2 mol%. Integrated genomic and phenotypic data supported the classification of strain JC271T as a representative of a novel genus, for which the name Halodesulfovibrio spirochaetisodalis gen. nov., sp. nov. is proposed. The type strain is JC271T (=KCTC 15474T=DSM 100016T). It is also proposed that Desulfovibrio acrylicus W218T is the latter heterotypic synonym of Desulfovibrio desulfuricans subsp. aestuarii Sylt 3T. Desulfovibrio desulfuricans subsp. aestuarii Sylt 3T should also be elevated as Halodesulfovibrio aestuarii comb. nov. and Desulfovibrio marinisediminisreclassified as Halodesulfovibrio marinisediminis comb. nov. Desulfovibrio oceani subsp. oceanishould be reclassified as Halodesulfovibrio oceani subsp. oceani comb. nov. and Desulfovibrio oceani subsp. galateae as Halodesulfovibrio oceani subsp. galateae comb. nov.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Água do Mar
/
Desulfovibrio
Tipo de estudo:
Prognostic_studies
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Índia