Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases.
Biotechnol Adv
; 35(1): 95-104, 2017.
Article
em En
| MEDLINE
| ID: mdl-28011075
ABSTRACT
The development of customizable sequence-specific nucleases such as TALENs, ZFNs and the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The CRISPR/Cas9 system is particularly versatile and has been applied in numerous species representing all branches of life. Regardless of the target organism, all researchers using sequence-specific nucleases face similar challenges confirmation of the desired on-target mutation and the detection of off-target events. Here, we evaluate the most widely-used methods for the detection of on-target and off-target mutations in terms of workflow, sensitivity, strengths and weaknesses.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Desoxirribonucleases
/
Sistemas CRISPR-Cas
/
Edição de Genes
/
Mutação
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biotechnol Adv
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha