Transcriptome analysis of Cucumis sativus infected by Cucurbit chlorotic yellows virus.
Virol J
; 14(1): 18, 2017 02 02.
Article
em En
| MEDLINE
| ID: mdl-28148297
ABSTRACT
BACKGROUND:
Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus-host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis.METHODS:
We used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection.RESULTS:
CCYV infection resulted in 865 DEGs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified 67 pathways, and the three major enrichment pathways (according to the P-values) were photosynthesis-antenna proteins (KO00196), phenylalanine metabolism (KO00360a), and phenylpropanoid biosynthesis (KO00940). Of the 13 DEGs identified in phenylalanine metabolism, 11 genes encode disease resistance-related phenylalanine ammonia-lyase (PAL) genes. Using quantitative real-time PCR, we validated the differential expression of 12 genes.CONCLUSIONS:
Our study based on the CCYV-cucumber interaction provides comprehensive transcriptomic information, and will improve our understanding of host-crinivirus interactions.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cucumis sativus
/
Perfilação da Expressão Gênica
/
Crinivirus
/
Interações Hospedeiro-Patógeno
Idioma:
En
Revista:
Virol J
Assunto da revista:
VIROLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China