Diverse and novel RHD variants in Australian blood donors with a weak D phenotype: implication for transfusion management.
Vox Sang
; 112(3): 279-287, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28220510
ABSTRACT
BACKGROUND AND OBJECTIVES:
Variant RHD genes associated with the weak D phenotype can result in complete or partial D-epitope expression on the red cell. This study examines the genetic classification in Australian blood donors with a weak D phenotype and correlates RHD variants associated with the weak D phenotype against D-epitope profile. MATERIALS ANDMETHODS:
Following automated and manual serology, blood samples from donors reported as 'weak D' (n = 100) were RHD genotyped by a commercial SNP-typing platform and Sanger sequencing. Two commercial anti-D antibody kits were used for extended serological testing for D-epitope profiles.RESULTS:
Three samples had wild-type RHD exonic sequences, and 97 samples had RHD variants. RHD*weak D type 1, RHD*weak D type 2 or RHD*weak D type 3 was detected in 75 donors. The remaining 22 samples exhibited 17 different RHD variants. One donor exhibited a novel RHD*c.939+3A>C lacking one D-epitope. Weak D types 1·1, 5, 15, 17 and 90 showed a partial D-epitope profile.CONCLUSION:
The array of RHD variants detected in this study indicated diversity in the Australian donor population that needs to be accommodated for in future genotyping strategies.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema do Grupo Sanguíneo Rh-Hr
/
Doadores de Sangue
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
País/Região como assunto:
Oceania
Idioma:
En
Revista:
Vox Sang
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Austrália