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Histone H1 defect in escort cells triggers germline tumor in Drosophila ovary.
Yang, Zhihao; Sun, Jin; Hu, Yuzhao; Wang, Fang; Wang, Xia; Qiao, Huan-Huan; Xu, Jiang; Mao, Decai; Ren, Xingjie; Pan, Li-Xia; Xu, Rong-Gang; Xu, Bo-Wen; Zhang, Yifan; Li, Haiyi; Miao, Wei; Hu, Yanhui; Chang, Zhijie; Wang, Dong; Li, Haitao; Chang, Zai; Liu, Lu-Ping; Liu, Qingfei; Ni, Jian-Quan.
Afiliação
  • Yang Z; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Sun J; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Hu Y; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Wang F; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Wang X; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Qiao HH; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Xu J; COFCO Nutrition and Health Research Institute, Beijing 102209, China; College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
  • Mao D; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Ren X; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Pan LX; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Xu RG; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Xu BW; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Zhang Y; Department of Biology, University of California, San Diego, USA.
  • Li H; French International School of Hong Kong, Hong Kong.
  • Miao W; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Hu Y; Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
  • Chang Z; School of Medicine, Institute of Biomedicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
  • Wang D; School of Medicine, Institute of Biomedicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
  • Li H; Department of Basic Medical Sciences, Center for Structural Biology, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Chang Z; School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Liu LP; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China.
  • Liu Q; School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China.
  • Ni JQ; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China. Electronic address: nijq@mail.tsinghua.edu.cn.
Dev Biol ; 424(1): 40-49, 2017 04 01.
Article em En | MEDLINE | ID: mdl-28232075
Drosophila ovary is recognized as one of the best model systems to study stem cell biology in vivo. We had previously identified an autonomous role of the histone H1 in germline stem cell (GSC) maintenance. Here, we found that histone H1 depletion in escort cells (ECs) resulted in an increase of spectrosome-containing cells (SCCs), an ovary tumor-like phenotype. Further analysis showed that the Dpp pathway is excessively activated in these SCC cells, while the expression of bam is attenuated. In the H1-depleted ECs, both transposon activity and DNA damage had increased dramatically, followed by EC apoptosis, which is consistent with the role of H1 in other somatic cells. Surprisingly, H1-depleted ECs acquired cap cell characteristics including dpp expression, and the resulting abnormal Dpp level inhibits SCC further differentiation. Most interestingly, double knockdown of H1 and dpp in ECs can reduce the number of SCCs to the normal level, indicating that the additional Dpp secreted by ECs contributes to the germline tumor. Taken together, our findings indicate that histone H1 is an important epigenetic factor in controlling EC characteristics and a key suppressor of germline tumor.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Histonas / Drosophila melanogaster / Células Germinativas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Dev Biol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Histonas / Drosophila melanogaster / Células Germinativas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Dev Biol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China