Uridylylation of the genome-linked protein of poliovirus in vitro is dependent upon an endogenous RNA template.
Virus Res
; 8(3): 193-204, 1987 Sep.
Article
em En
| MEDLINE
| ID: mdl-2825442
ABSTRACT
The properties of an in vitro replication system derived from a membrane fraction (crude replication complex, CRC) of poliovirus-infected HeLa cells were examined. This system was capable of producing the nucleotidyl-proteins VPg-pU and VPg-pUpU. Due to high intrinsic phosphoesterase(s) activity and endogenous nucleoside triphosphate pools the yield of labeled product was low. Treatment of CRC with DEAE-cellulose and addition of an ATP generating system resulted in a dramatic increase in the level of nucleotidyl-proteins formed. The capacity to form VPg-pU and VPg-pUpU could be completely abolished by pretreatment of CRC with nuclease, an observation suggesting that the uridylylation of VPg is a template-dependent reaction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uridina
/
Proteínas Virais
/
RNA Viral
/
Poliovirus
/
Genes Virais
Limite:
Humans
Idioma:
En
Revista:
Virus Res
Assunto da revista:
VIROLOGIA
Ano de publicação:
1987
Tipo de documento:
Article