Eukaryotic DNA Replication Fork.
Annu Rev Biochem
; 86: 417-438, 2017 06 20.
Article
em En
| MEDLINE
| ID: mdl-28301743
ABSTRACT
This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
DNA Helicases
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DNA Polimerase II
/
Replicação do DNA
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Células Eucarióticas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Annu Rev Biochem
Ano de publicação:
2017
Tipo de documento:
Article