Plant glutamine synthetase complements a glnA mutation in Escherichia coli.
Science
; 232(4755): 1242-4, 1986 Jun 06.
Article
em En
| MEDLINE
| ID: mdl-2871626
ABSTRACT
A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Recombinante
/
Medicago sativa
/
Escherichia coli
/
Glutamato-Amônia Ligase
Idioma:
En
Revista:
Science
Ano de publicação:
1986
Tipo de documento:
Article