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Solanum paniculatum L. decreases levels of inflammatory cytokines by reducing NFKB, TBET and GATA3 gene expression in vitro.
Rios, Raimon; Silva, Hugo Bernardino Ferreira da; Carneiro, Norma Vilany Queiroz; Pires, Anaque de Oliveira; Carneiro, Tamires Cana Brasil; Costa, Ryan Dos Santos; Marques, Cintia Rodrigues; Machado, Marta Santos Serafim; Velozo, Eudes da Silva; Silva, Telma M G da; Silva, Tania M S da; Conceição, Adilva de Souza; Alcântara-Neves, Neuza Maria; Figueiredo, Camila Alexandrina.
Afiliação
  • Rios R; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Silva HBFD; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Carneiro NVQ; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Pires AO; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Carneiro TCB; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Costa RDS; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Marques CR; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Machado MSS; Faculdade Adventista da Bahia, BR 101, km 197, 44300000, Capoeiruçu, Cachoeira, Bahia, Brazil.
  • Velozo EDS; Departamento do Medicamento, Faculdade de Farmácia, Universidade Federal da Bahia, Campus Ondina, 40170115 Salvador, Bahia, Brazil.
  • Silva TMGD; Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, 52171900 Recife, Pernambuco, Brazil.
  • Silva TMSD; Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, 52171900 Recife, Pernambuco, Brazil.
  • Conceição AS; Departamento de Educação, Universidade do Estado da Bahia, Campus VIII, 48608240 Paulo Afonso, Bahia, Brazil.
  • Alcântara-Neves NM; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil.
  • Figueiredo CA; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Campus Canela, 40110100 Salvador, Bahia, Brazil. Electronic address: cavfigueiredo@gmail.com.
J Ethnopharmacol ; 209: 32-40, 2017 Sep 14.
Article em En | MEDLINE | ID: mdl-28729226
ETHNOPHARMACOLOGICAL RELEVANCE: Solanum paniculatum L., popularly known as jurubeba, is a common subtropical plant from Brazil, Paraguay, Bolivia and Argentina, that is used in folk medicine for the treatment of anemia, gastrointestinal disorders and inflammatory conditions in general. In addition to that, an ethnobotanical survey in "Todos os Santos" Bay have pointed out S. paniculatum as an herb to treat asthma. Previous publications have shown that S. paniculatum possesses antibiotic, antioxidant and modulatory effects on gastric acid secretion; however, its anti-inflammatory potential remains unexplored. AIM OF THE STUDY: Herein, we analyzed the S. paniculatum fruits hexane extract (SpE) for the presence of stigmasterol and ß-sitosterol and investigated the anti-inflammatory effect of SpE in vitro. MATERIALS AND METHODS: SpE was subjected to high-performance liquid chromatography (HPLC) for standardization and quantification of stigmasterol and ß-sitosterol. Spleen cells from BALB/c mice were cultivated and stimulated with pokeweed mitogen and also exposed to 15, 30 and 60µg/mL of SpE. Following treatment, levels of IFN-γ, IL-4 and IL-10 in the culture supernatants were assessed by ELISA. We also evaluated nitric oxide (NO) production by murine LPS-stimulated peritoneal macrophages using the Griess technique. In addition, the ability of SpE to stabilize membranes was assessed using a model of hemolysis induced by heat on murine erythrocytes. Gene expression of Th1-cell-specific Tbx21 transcription factor (TBET), zinc-finger transcription factor-3 (GATA3), and nuclear factor-κB (NFKB) in murine spleen cells were assessed by quantitative Polymerase Chain Reaction (qRT-PCR). RESULTS: SpE at 15, 30 and 60µg/mL significantly attenuated cell proliferation, decreased IL-4 release, reduced NO production and improved erythrocyte membrane stabilization in a concentration-dependent manner. SpE was also able to decrease the release of IFN-γ without altering IL-10 levels. The mechanism whereby SpE decreased inflammatory markers may be related to the reduction of NFKB, TBET and GATA3 gene expression. CONCLUSIONS: This study is the first to test the anti-inflammatory action of S. paniculatum. Herein, we provided evidence for the popular use of S. paniculatum in inflammatory conditions. Additional studies must be conducted to further explore the anti-inflammatory potential of SpE and to elucidate possible clinical applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Citocinas / Solanum / Inflamação Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Citocinas / Solanum / Inflamação Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil