Peptides derived from tryptic hydrolysate of Bacillus subtilis culture suppress fungal spoilage of table grapes.
Food Chem
; 239: 520-528, 2018 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-28873599
ABSTRACT
This study confirmed the anti-fungal effect of trypsin-treated Bacillus subtilis culture (BC) (tryptic hydrolysate, TH) on mold growth on Kyoho grapes. We examined the anti-fungal activity of TH by identifying TH peptides and performing a computational docking analysis. TH was more potent than untreated BC in suppressing fungal growth on grapes. Specifically, TH maintained grape freshness by inhibiting respiration and rachis browning, maintaining firmness, and preventing weight loss. Thirty-six inhibitory peptides against ß-1,3-glucan synthase (GS) were screened from 126 TH peptides identified through proteomic analysis. Among them, 13 peptides bound tightly to GS active pockets with lower binding energies than that of GppNHp. The most potent peptides, LFEIDEELNEK and FATSDLNDLYR, were synthesized, and further experiments showed that these peptides had a highly suppressive effect on GS activity and Aspergillus niger and Penicillium chrysogenum growth. Our results confirm that tryptic treatment is effective for improving the anti-fungal activity of BC.
Palavras-chave
Acetonitrile (PubChem CID: 6342); Browning; Chitosan (PubChem CID: 71853); Computational docking; Formic acid (PubChem CID: 284); GTPase; Glucose (PubChem CID: 79025); GppNHp (PubChem CID: 36735); Molds; Respiratory rate; Sodium acetate (PubChem CID: 517045); Sodium chloride (PubChem CID: 5234); Sodium hydroxide (PubChem CID: 14798); Sulfur dioxide (PubChem CID: 1119); Trypsin (PubChem CID: 72699210); ß-1,3-Glucan synthase
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China