Discovery of New Glycosidases From Metagenomic Libraries.
Methods Enzymol
; 597: 3-23, 2017.
Article
em En
| MEDLINE
| ID: mdl-28935108
ABSTRACT
Microorganisms routinely perform complex enzymatic transformations of natural material; thus, their enzymes have the potential to tackle medical and industrial challenges. However, a vast number of microbes are recalcitrant to cultivation. Functional screening of environmental DNA allows us to tap into the seemingly boundless diversity of enzymes encoded by microbial populations. In this chapter, we describe methods for the isolation of environmental DNA, generation of metagenomic libraries, and the functional screening of these libraries for new glycosidases.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
DNA Bacteriano
/
Metagenômica
/
Glicosídeo Hidrolases
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Canadá