Stimulation of IL-1ß and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis.
Parasit Vectors
; 10(1): 445, 2017 Sep 26.
Article
em En
| MEDLINE
| ID: mdl-28950910
ABSTRACT
BACKGROUND:
Angiostrongylus cantonensis is an important causative agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. Previous studies have shown that the Sonic hedgehog (Shh) signaling pathway may reduce cell apoptosis by inhibiting oxidative stress in A. cantonensis infection. In this study, we investigated the relationship between cytokine secretion and Shh pathway activation after treatment with excretory/secretory products (ESP) of fifth-stage larval A. cantonensis (L5).RESULTS:
The results showed that IL-1ß and IL-6 levels in mouse astrocytes were increased. Moreover, ESP stimulated the protein expression of Shh pathway molecules, including Shh, Ptch, Smo and Gli-1, and induced IL-1ß and IL-6 secretion. The transcription factor nuclear factor-κB (NF-κB) plays an important role in inflammation, and it regulates the expression of proinflammatory genes, including cytokines and chemokines, such as IL-1ß and TNF-α. After ESP treatment, NF-κB induced IL-1ß and IL-6 secretion in astrocytes by activating the Shh signaling pathway.CONCLUSIONS:
Overall, the data presented in this study showed that ESP of fifth-stage larval A. cantonensis stimulates astrocyte activation and cytokine generation through NF-κB and the Shh signaling pathway.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Helminto
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Astrócitos
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NF-kappa B
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Interleucina-6
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Infecções por Strongylida
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Angiostrongylus cantonensis
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Interleucina-18
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Proteínas Hedgehog
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Parasit Vectors
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Taiwan