Andrographolide relieved pathological pain generated by spared nerve injury model in mice.
Pharm Biol
; 56(1): 124-131, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-29385888
ABSTRACT
CONTEXT Andrographolide (Andro), found in large quantities in Andrographis paniculata Nees (Acanthaceae), is anti-inflammatory, especially in the central nervous system (CNS) glia. OBJECTIVE:
The objective of this study is to test Andro's ability to reduce allodynia in a spared nerve injury model. MATERIAL ANDMETHODS:
Male 30 g BalbC mice were divided into four groups (1) Sham-operated control (Sham-group); (2) nerve injured and treated with saline (Saline-group); (3) nerve injured and treated with Andro (Andro-group); (4) nerve injured and treated with non-steroidal anti-inflammatory drugs (NSAIDS) (NSAIDS-group). Andro or NSAIDS (diclofenac salt) were injected intraperitoneally at 5 mg/kg body weight daily. Mechanical allodynia was assessed by von Frey tests at 3, 7, and 14 d. For immunohistochemical analysis, samples were collected at 7 d.RESULTS:
The threshold for inducing allodynia increased and the response percentage reduced in the Andro-group when compared with the Saline-group, as well as when compared with NSAIDS groups throughout 3-14 d. The ratio of threshold for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS groups was 20.42 and 11.67 at 14 d, respectively. The ratio of response percentage for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS was 0.32 and 0.39 at 14 d, respectively. Interleukin-1 (IL-1) immunostaining in the spinal cord was reduced in the Andro-group. Astrocytic activities were not significantly reduced in the Andro-group compared with the Saline-group at 7 d post-operation (PO)Conclusions:
Andro reduced mechanical allodynia more than NSAIDS at the same concentration, and the observed behaviour was associated with a reduction in inflammatory cytokine produced in the spinal cord.Palavras-chave
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dor
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Modelos Animais de Doenças
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Diterpenos
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Hiperalgesia
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Anti-Inflamatórios
Limite:
Animals
Idioma:
En
Revista:
Pharm Biol
Ano de publicação:
2018
Tipo de documento:
Article