Six-month cultured cerebral organoids from human ES cells contain matured neural cells.
Neurosci Lett
; 670: 75-82, 2018 03 23.
Article
em En
| MEDLINE
| ID: mdl-29398520
ABSTRACT
Recently, researchers have developed protocols for human cerebral organoids using human pluripotent stem cells, which mimic the structure of the developing human brain. Existing research demonstrated that human cerebral organoids which undergo short cultivation periods, contain astrocytes, neurons, and neural stem cells, but lacked mature oligodendrocytes, and mature, fully functional neurons. In this study, we analyzed organoids induced from H9 human embryonic stem (ES) cells that were cultivated for as long as six months. We observed mature oligodendrocytes, positive for MBP (myelin-basic protein), and mature GAD67 (glutamate decarboxylase 67â¯kDa isoform)-positive inhibitory neurons and VGLUT1 (vesicular glutamate transporter 1)-positive excitatory neurons via immunohistochemical analysis. These observations suggest that long-term cultivation of cerebral organoids can lead to the maturation of human cerebral organoids, which can be used as a tool to study the development of human brains.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Organoides
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Oligodendroglia
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Neurogênese
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Células-Tronco Neurais
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Células-Tronco Embrionárias Humanas
Limite:
Humans
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2018
Tipo de documento:
Article