Immunolocalization of Receptor and Chemoreceptor Modules in the Sheep Vomeronasal Organ.

ABSTRACT

The vomeronasal organ (VNO) is the peripheral receptor organ of the accessory olfactory system, which is responsible for both sexual and innate behaviors. The degree of neuronal differentiation and maturation of the vomeronasal receptor cells together with the verification of the presence of the solitary chemoreceptor cells (SCCs) in the VNO of Corriedale sheep were assessed using immunofluorescence. A protein gene product 9.5 (PGP 9.5), which is a neuronal marker recognized to be expressed in most neurons of vertebrate species, an olfactory marker protein (OMP) that is precise for mature olfactory receptor cells, and lastly phospholipase C-ß2 (PLC-ß2), a marker in the signal transduction pathway of SCCs, were all tested. The cell bodies and dendrites of almost all receptor cells in the sensory epithelium were strongly positive for PGP 9.5 and to a lesser extent for OMP. In the nonsensory wall, all cells were negative for both PGP 9.5 and OMP; however, some positive PGP 9.5 immunoreactive fibers were identified. For PLC-ß2, only 1 basally situated SCC could be identified in the sensory epithelium. A higher number was demonstrated in the nonsensory wall. Corriedale sheep possess matured, fully differentiated vomeronasal receptor cells in their sensory wall, suggesting an appropriate pheromone perception. Additionally, the VNO in sheep may participate in the usual transduction mechanisms, though it is seemingly not a chemoreceptor organ.