Effect of electron beam and gamma radiation on drug-susceptible and drug-resistant Listeria monocytogenes strains in salmon under different temperature.
J Appl Microbiol
; 125(3): 828-842, 2018 Sep.
Article
em En
| MEDLINE
| ID: mdl-29727511
ABSTRACT
AIMS:
To investigate the effect of gamma radiation and high energy electron beam doses on the inactivation of antibiotic-susceptible and antibiotic-resistant Listeria monocytogenes strains inoculated on the surface of raw salmon fillets stored at different temperature (-20, 4 and 25°C). METHODS ANDRESULTS:
The population of bacteria strains resistance to penicillin, ampicillin, meropenem, erythromycin and trimethoprim-sulfamethoxazole was generated. When using gamma irradiation, the theoretical lethal dose ranged from 1·44 to 5·68 kGy and for electron beam the values ranged from 2·99 to 6·83 kGy. The theoretical lethal dose for both radiation methods was higher for antibiotic-resistant strains. Gamma radiation proved to be a more effective method for extending salmon fillet shelf-life. The evaluation of pulsed-field gel electrophoresis electrophoregram revealed that the repair of radiation-caused DNA damage occurred faster in antibiotic-resistant L. monocytogenes strains. The number of live L. monocytogenes cells, 40 h after irradiation, also was higher in antibiotic-resistant strain suspension.CONCLUSIONS:
The present study showed that gamma radiation was more effective in the elimination of the tested micro-organisms and food preservation, than a high energy electron beam. The antibiotic-resistant L. monocytogenes strains were more resistant to both radiation methods. SIGNIFICANCE AND IMPACT OF THE STUDY There are a lot of research on the effect of radiation on the number of bacteria in food products. However, there is almost no information about the effect of strain properties, such as drug susceptibility, virulence, etc., on their resistance to ionizing radiation. An increasing number of drug resistant bacterial strains isolated from food, encourages to take up this research subject.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmão
/
Alimentos Marinhos
/
Farmacorresistência Bacteriana
/
Irradiação de Alimentos
/
Listeria monocytogenes
Limite:
Animals
Idioma:
En
Revista:
J Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Polônia