Dextrancoated superparamagnetic iron oxide nanoparticles activate the MAPK pathway in human primary monocyte cells.
Mol Med Rep
; 18(1): 564-570, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29749448
ABSTRACT
With the increase in applications of superparamagnetic iron oxide nanoparticles (SPIONs) in biomedicine, it is essential to investigate the biosecurity of these nanoparticles, especially with respect to the human immune system. In the present study, the biological effects of dextrancoated superparamagnetic iron oxide nanoparticles (DexSPIONs) on human primary monocyte cells were evaluated. The results of the present study demonstrated that DexSPIONs can be identified in phagosomes or freed in the cytoplasm and did not affect cell viability or induce apoptosis. Notably, there were certain bulky vacuoles and a number of pseudopodia from the cell membrane, suggesting potential activation of human monocyte cells. In addition, the expression levels of proinflammatory cytokines interleukin (IL)1ß and tumor necrosis factor (TNF)α were also increased following treatment with DexSPIONs. Simultaneously, the phosphorylation levels of mitogenactivated protein kinase (MAPK) p38, cJun Nterminal kinase 1 and extracellular signal regulated kinase were markedly enhanced following nanoparticle exposure and MAPK inhibitors could abate the production of IL1ß and TNFα. The results of the present study demonstrated that DexSPIONs could activate human monocyte cells and that activation of MAPK pathway may be involved in these effects.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
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Dextranos
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Fator de Necrose Tumoral alfa
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Sistema de Sinalização das MAP Quinases
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Interleucina-1beta
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Nanopartículas de Magnetita
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2018
Tipo de documento:
Article