Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine.
Science
; 228(4702): 958-62, 1985 May 24.
Article
em En
| MEDLINE
| ID: mdl-2988125
ABSTRACT
The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficiently expressed in the bacterium Escherichia coli. When injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic CS protein on live sporozoites and blocked sporozoite invasion of human hepatoma cells in vitro. These CS protein derivatives are therefore candidates for a human malaria vaccine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Vacinas
/
Proteínas de Protozoários
/
Malária
/
Formação de Anticorpos
/
Antígenos de Superfície
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1985
Tipo de documento:
Article