Effect of seaweed supplementation on tocopherol concentrations in bovine milk using dispersive liquid-liquid microextraction.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1092: 152-157, 2018 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-29902754
ABSTRACT
A dispersive liquid-liquid microextraction (DLLME) method, combined with HPLC-UV detection, was developed for the extraction and preconcentration of δ-tocopherol from bovine milk. This method was used to study the effect of supplementing cow feed with the seaweed Ascophyllum nodosum on vitamin content in milk. The optimal experimental conditions were determined 200⯵L of chloroform (extraction solvent), 1.0â¯mL of ethanol (dispersive solvent), 5â¯mL of water (aqueous phase). Under these optimal conditions the DLLME method provided linearity in the range 0.01⯵g/mL to 8⯵g/mL with R2 values of 0.998. Limit of detection (LOD) was 0.01⯵g/mL, while the enrichment factor was 89. Cow feed that was supplemented with Ascophyllum nodosum was shown to increase δ-tocopherol levels from 3.82⯵g/mL to 5.96⯵g/mL.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alga Marinha
/
Suplementos Nutricionais
/
Tocoferóis
/
Leite
/
Microextração em Fase Líquida
/
Ração Animal
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2018
Tipo de documento:
Article