Effect of Deltex-1 on proliferation and differentiation of bone marrow mesenchymal stem cells into smooth muscle cells.
Eur Rev Med Pharmacol Sci
; 22(12): 3627-3634, 2018 06.
Article
em En
| MEDLINE
| ID: mdl-29949133
ABSTRACT
OBJECTIVE:
To investigate the effect of Deltex-1 on proliferation and differentiation of bone marrow mesenchymal stem cells (bMSCs) into smooth muscle cells (SMCs). MATERIALS ANDMETHODS:
bMSCs of rat were isolated from bone marrow, cultured and identified. The effect of Deltex-1 on the proliferation of bMSCs infected with adenovirus vector pAd/Deltex-1 was detected by cell count kit-8 (CCK-8). The expression of smooth muscle myosin heavy chain (SM-MHC), that is one of the markers of SMCs in bMSCs without treatment, with Deltex-1 virus infection or empty virus infection and co-cultured with SMCs, were detected by immunofluorescence cytochemistry staining, RT-PCR and Western blotting. The same detection of bMSCs and SMCs without treatment was used as normal control, respectively.RESULTS:
bMSCs of rat were isolated from bone marrow, cultured and identified. Compared with the control, the results of CCK-8 showed that the growth of bMSCs infected by Deltex-1 virus was slower, and began to appear more significant especially at 48 (p<0.05, p<0.01). The results of immunofluorescence cytochemistry, Real-time PCR and Western blot showed that bMSCs with Deltex-1 virus infection and co-cultured with SMCs significantly expressed SM-MHC, and weakly expressed Notch-1.CONCLUSIONS:
The proliferation of bMSCs with Deltex-1 over-expression could be inhibited and its differentiation into smooth muscle cells could be promoted.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Miócitos de Músculo Liso
/
Ubiquitina-Proteína Ligases
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China