Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells.

Martignani, Eugenio; Accornero, Paolo; Miretti, Silvia; Baratta, Mario

Martignani, Eugenio; Accornero, Paolo; Miretti, Silvia; Baratta, Mario.

Afiliação

Martignani E; Department of Veterinary Science, University of Turin, Grugliasco, TO, Italy. eugenio.martignani@unito.it.
Accornero P; Department of Veterinary Science, University of Turin, Grugliasco, TO, Italy.
Miretti S; Department of Veterinary Science, University of Turin, Grugliasco, TO, Italy.
Baratta M; Department of Veterinary Science, University of Turin, Grugliasco, TO, Italy.

Methods Mol Biol ; 1817: 137-144, 2018.

Article em En | MEDLINE | ID: mdl-29959710

ABSTRACT

ABSTRACT

Bovine mammary organoids are cell aggregates that are produced by an association of a mechanical and an enzymatic dissociation of mammary gland tissue. They provide a useful source to isolate mammary epithelial cells, but can also be frozen as an intermediate dissociation step.Due to the strong cell-cell interactions among epithelial cells, the production and isolation of organoids is an efficient way to remove unwanted cell population of non-epithelial origin like fibroblasts.

Assuntos

Glândulas Mamárias Animais/citologia; Organoides/citologia; Técnicas de Cultura de Tecidos/métodos; Animais; Bovinos; Células Epiteliais/citologia; Feminino; Modelos Biológicos

Palavras-chave

Bovine; Dissociation; Epithelial cells; Extracellular matrix; Mammary gland; Organoids

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Organoides / Técnicas de Cultura de Tecidos / Glândulas Mamárias Animais Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Itália

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