Evidence for genetic divergence in ribosomal RNA genes in mycobacteria.
FEBS Lett
; 195(1-2): 194-8, 1986 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-3002853
ABSTRACT
DNA was isolated from Mycobacterium phlei and from M. smegmatis. Each DNA sample was restricted with endonucleases, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose film. Fragments of DNA containing rRNA sequences were identified by means of 125I-labelled rRNA of M. phlei or of M. smegmatis. The distributions of restriction endonuclease sites within the rRNA gene(s) and flanking sequences were found to be characteristic for each of the two species. Hybridizations with heterologous probes indicate that although M. phlei rRNA and M. smegmatis rRNA share regions of sequence homology, they are probably not identical in primary structure. The results suggest that the rRNA genes might prove to be useful taxonomic markers for mycobacteria.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Ribossômico
/
RNA Ribossômico
/
Mycobacterium
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1986
Tipo de documento:
Article