Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.
Appl Environ Microbiol
; 51(1): 206-8, 1986 Jan.
Article
em En
| MEDLINE
| ID: mdl-3006586
ABSTRACT
A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent transposition events inactivated the sulfometuron methyl resistance determinant, localizing the ILV2 gene to a minimum 1.4-kilobase-pair region.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leveduras
/
Elementos de DNA Transponíveis
/
Mapeamento Cromossômico
/
Genes Fúngicos
/
Mutação
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
1986
Tipo de documento:
Article