Effects of donor age, donor sex, blood-component processing, and storage on cell-derived microparticle concentrations in routine blood-component preparation.
Transfus Apher Sci
; 57(4): 587-592, 2018 Aug.
Article
em En
| MEDLINE
| ID: mdl-30082165
ABSTRACT
BACKGROUND:
A number of factors cause increases in the number of cell-derived microparticles (MPs) in blood components. However, the overall effects of these factors on the concentration of MPs during routine blood-component preparation have not fully been elucidated.AIM:
To evaluate the effects of donor age, donor sex, blood-component preparation, and storage on MP concentrations.METHODS:
Flow cytometry was used to quantitate the number of whole blood-derived MPs.RESULTS:
The total MP concentration was similar in male and female donors (26,044 ± 1254 particles/µL vs. 27,696 ± 1584 particles/µL). The total MP concentration did not differ significantly among the different age groups 18-30 years (28,730 ± 1600 particles/µL), 31-40 years (24,972 ± 5947 particles/µL), and 41-58 years (25,195 ± 1727 particles/µL). However, the total number of MPs in fresh plasma (152,110 ± 46,716 particles/µL) was significantly higher (p < 0.05) than that in unprocessed whole blood (26,752 ± 985 particles/µL), fresh packed red blood cells (PRBCs) (28,574 ± 1028 particles/µL), and platelet concentrate (PC) (33,072 ± 1858 particles/µL). Furthermore, the total numbers of MPs in stored PRBCs and fresh-frozen plasma (FFP) were significantly higher (p < 0.05) than those in fresh PRBCs and fresh plasma, respectively.CONCLUSIONS:
The study suggests that donor factors, blood-component processing and storage contribute to the MP concentration in routine blood-product preparation. The findings can improve quality control and management of blood-product manufacturing in routine transfusion laboratories.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Preservação de Sangue
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Transfusão de Componentes Sanguíneos
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Micropartículas Derivadas de Células
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Transfus Apher Sci
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article