Pumpkin CmHKT1;1 Controls Shoot Na⺠Accumulation via Limiting Na⺠Transport from Rootstock to Scion in Grafted Cucumber.
Int J Mol Sci
; 19(9)2018 Sep 06.
Article
em En
| MEDLINE
| ID: mdl-30200653
ABSTRACT
Soil salinity adversely affects the growth and yield of crops, including cucumber, one of the most important vegetables in the world. Grafting with salt-tolerant pumpkin as the rootstock effectively improves the growth of cucumber under different salt conditions by limiting Na⺠transport from the pumpkin rootstock to the cucumber scion. High-affinity potassium transporters (HKTs) are crucial for the long distance transport of Na⺠in plants, but the function of pumpkin HKTs in this process of grafted cucumber plants remains unclear. In this work, we have characterized CmHKT1;1 as a member of the HKT gene family in Cucurbita moschata and observed an obvious upregulation of CmHKT1;1 in roots under NaCl stress conditions. Heterologous expression analyses in yeast mutants indicated that CmHKT1;1 is a Naâº-selective transporter. The transient expression in tobacco epidermal cells and in situ hybridization showed CmHKT1;1 localization at plasma membrane, and preferential expression in root stele. Moreover, ectopic expression of CmHKT1;1 in cucumber decreased the Na⺠accumulation in the plants shoots. Finally, the CmHKT1;1 transgenic line as the rootstock decreased the Na⺠content in the wild type shoots. These findings suggest that CmHKT1;1 plays a key role in the salt tolerance of grafted cucumber by limiting Na⺠transport from the rootstock to the scion and can further be useful for engineering salt tolerance in cucurbit crops.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Sódio
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Cucumis sativus
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Cucurbita
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Proteínas de Transporte de Cátions
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Simportadores
Idioma:
En
Revista:
Int J Mol Sci
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China