Observational Study Design in Veterinary Pathology, Part 2: Methodology.
Vet Pathol
; 55(6): 774-785, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30227783
ABSTRACT
Observational studies are a basis for much of our knowledge of veterinary pathology, yet considerations for conducting pathology-based observational studies are not readily available. In part 1 of this series, we offered advice on planning and carrying out an observational study. Part 2 of the series focuses on methodology. Our general recommendations are to consider using already-validated methods, published guidelines, data from primary sources, and quantitative analyses. We discuss 3 common methods in pathology research-histopathologic scoring, immunohistochemistry, and polymerase chain reaction-to illustrate principles of method validation. Some aspects of quality control include use of clear objective grading criteria, validation of key reagents, assessing sample quality, determining specificity and sensitivity, use of technical and biologic negative and positive controls, blinding of investigators, approaches to minimizing operator-dependent variation, measuring technical variation, and consistency in analysis of the different study groups. We close by discussing approaches to increasing the rigor of observational studies by corroborating results with complementary methods, using sufficiently large numbers of study subjects, consideration of the data in light of similar published studies, replicating the results in a second study population, and critical analysis of the study findings.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Patologia Veterinária
/
Estudos Observacionais como Assunto
Tipo de estudo:
Observational_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Pathol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Canadá