Characterization of rat schwannoma-Schwann cell hybrids.
Brain Res
; 397(2): 238-44, 1986 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-3026558
ABSTRACT
Sciatic nerve Schwann cells from strain LEC rats, homozygous for the c form of 6-phosphogluconate dehydrogenase (6-PGD), and RN22 rat Schwannoma cells, a subclone of RN2 deficient in hypoxanthine phosphoribosyltransferase and expressing the s form of 6-PGD, were fused to produce 'RNS' hybrid clones which proliferate rapidly in a medium containing hypoxanthine, aminopterin and thymidine (HAT) and express c, s and c/s heterodimeric forms of 6-PGD. RNS cells, like both parents, maintain a high baseline activity of 2', 3'-cyclic nucleotide 3'-phosphohydrolase and, as in RN22, activity of this enzyme is further inducible by 1 mM N6, O2'-dibutyryl 3', 5'-cyclic AMP. The RNS clones resemble normal Schwann cells in the capacity to bind radioiodinated axolemmal fragments to their plasma membranes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células de Schwann
/
Células Híbridas
/
Neurilemoma
Limite:
Animals
Idioma:
En
Revista:
Brain Res
Ano de publicação:
1986
Tipo de documento:
Article