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Modified Rat Hepatocellular Carcinoma Models Overexpressing Vascular Endothelial Growth Factor.
Choi, Jin Woo; Cho, Hye Rim; Lee, Kyoungbun; Jung, Jae Kyung; Kim, Hyo-Cheol.
Afiliação
  • Choi JW; Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
  • Cho HR; Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
  • Lee K; Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
  • Jung JK; Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea.
  • Kim HC; Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea. Electronic address: angiointervention@gmail.com.
J Vasc Interv Radiol ; 29(11): 1604-1612, 2018 11.
Article em En | MEDLINE | ID: mdl-30293733
ABSTRACT

PURPOSE:

To compare tumor vascularity in 4 types of rat hepatocellular carcinoma (HCC) models N1S1, vascular endothelial growth factor (VEGF)-transfected N1S1 (VEGF-N1S1), McA-RH7777, and VEGF-transfected McA-RH7777 (VEGF-McA-RH777) tumors. MATERIALS AND

METHODS:

The N1S1 and McA-RH7777 cell lines were transfected with expression vectors containing cDNA for rat VEGF. Eighty-eight male Sprague-Dawley rats (weight range, 400-450 g) were randomly divided into 4 groups (ie, 22 rats per model), and 4 types of tumor models were created by using the N1S1, VEGF-N1S1, McA-RH7777, and VEGF-McA-RH777 cell lines. Tumor vascularity was evaluated by perfusion computed tomography (CT), enzyme-linked immunosorbent assay of VEGF, CD34 staining, angiography, and Lipiodol transarterial embolization. Intergroup discrepancies were evaluated by Kruskal-Wallis test.

RESULTS:

Arterial perfusion (P < .001), portal perfusion (P = .015), total perfusion (P < .001), tumor VEGF level (P = .002), and microvessel density (MVD; P = .007) were significantly different among groups. VEGF-McA-RH7777 tumors showed the greatest arterial perfusion (46.7 mL/min/100 mL ± 15.5), total perfusion (60.7 mL/min/100 mL ± 21.8), tumor VEGF level (3,376.7 pg/mL ± 145.8), and MVD (34.5‰ ± 7.5). Whereas most tumors in the N1S1, VEGF-N1S1, and McA-RH7777 groups showed hypovascular staining on angiography and minimal Lipiodol uptake after embolization, 5 of 6 VEGF-McA-RH7777 tumors (83.3%) presented hypervascular tumor staining and moderate to compact Lipiodol uptake.

CONCLUSIONS:

McA-RH7777 tumors were more hypervascular than N1S1 tumors, and tumor vascularity was enhanced further by VEGF transfection. Therefore, the VEGF-McA-RH7777 tumor is recommended to mimic hypervascular human HCC in rats.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Fator A de Crescimento do Endotélio Vascular / Neoplasias Hepáticas Experimentais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Vasc Interv Radiol Assunto da revista: ANGIOLOGIA / RADIOLOGIA Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Fator A de Crescimento do Endotélio Vascular / Neoplasias Hepáticas Experimentais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Vasc Interv Radiol Assunto da revista: ANGIOLOGIA / RADIOLOGIA Ano de publicação: 2018 Tipo de documento: Article