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SrcA is a chaperone for the Salmonella SPI-2 type three secretion system effector SteD.
Godlee, Camilla; Cerny, Ondrej; Durkin, Charlotte H; Holden, David W.
Afiliação
  • Godlee C; MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK.
  • Cerny O; MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK.
  • Durkin CH; MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK.
  • Holden DW; MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK.
Microbiology (Reading) ; 165(1): 15-25, 2019 01.
Article em En | MEDLINE | ID: mdl-30457515
Effector proteins of type three secretion systems (T3SS) often require cytosolic chaperones for their stabilization, to interact with the secretion machinery and to enable effector delivery into host cells. We found that deletion of srcA, previously shown to encode a chaperone for the Salmonella pathogenicity island 2 (SPI-2) T3SS effectors SseL and PipB2, prevented the reduction of mature Major Histocompatibility Complex class II (mMHCII) from the surface of antigen-presenting cells during Salmonella infection. This activity was shown previously to be caused by the SPI-2 T3SS effector SteD. Since srcA and steD are located in the same operon on the Salmonella chromosome, this suggested that the srcA phenotype might be due to an indirect effect on SteD. We found that SrcA is not translocated by the SPI-2 T3SS but interacts directly and forms a stable complex with SteD in bacteria with a 2 : 1 stoichiometry. We found that SrcA was not required for SPI-2 T3SS-dependent, neutral pH-induced secretion of either SseL or PipB2 but was essential for secretion of SteD. SrcA therefore functions as a chaperone for SteD, explaining its requirement for the reduction in surface levels of mMHCII.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Proteínas de Bactérias / Chaperonas Moleculares / Ilhas Genômicas / Sistemas de Secreção Tipo III Limite: Humans Idioma: En Revista: Microbiology (Reading) Assunto da revista: MICROBIOLOGIA Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Proteínas de Bactérias / Chaperonas Moleculares / Ilhas Genômicas / Sistemas de Secreção Tipo III Limite: Humans Idioma: En Revista: Microbiology (Reading) Assunto da revista: MICROBIOLOGIA Ano de publicação: 2019 Tipo de documento: Article