Application of High-throughput Sequencing in Researches of Cadaveric Microorganisms and Postmortem Interval Estimation.
Fa Yi Xue Za Zhi
; 34(5): 475-481, 2018 10.
Article
em Zh, En
| MEDLINE
| ID: mdl-30468048
ABSTRACT
Necrobiome is the main factor causing the cadaver decomposition. Studying the microbial succession during decomposition is one of the main tasks of forensic microbiology. The interactive relationships among cadaver, environment and microorganisms are complicated. The microbial succession study relies on macroscopic monitoring of community composition and the diversity change in each decomposition stage. With the maturity and development of high-throughput sequencing ï¼HTSï¼, the structure and diversity of microbial communities in different environments have been successively revealed. A new breakthrough to explore the cadaveric microorganisms has been opened as well. It has become the research hotspots in forensic microbiology to reveal the microbial succession in the process of cadaver decomposition and to interpret the essence of various decomposition phenomena by using HTS, which can provide a new reference for postmortem interval ï¼PMIï¼ estimation. The present paper reviews studies on PMI estimation by using cadaveric microorganism. Problems and application prospects of forensic microbiology studies are discussed on the basis of the current application of HTS technology in the exploration of microbial succession.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mudanças Depois da Morte
/
Bactérias
/
Sequenciamento de Nucleotídeos em Larga Escala
Limite:
Humans
Idioma:
En
/
Zh
Revista:
Fa Yi Xue Za Zhi
Assunto da revista:
JURISPRUDENCIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China