Acanthamoeba proteases contribute to macrophage activation through PAR1 , but not PAR2.
Parasite Immunol
; 41(2): e12612, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30578557
ABSTRACT
AIM:
Acanthamoeba infections are characterized by an intense localized innate immune response associated with an influx of macrophages. Acanthamoeba protease production is known to affect virulence. Herein, the ability of Acanthamoeba trophozoite proteases, of either the laboratory Neff strain or a recently isolated clinical strain, to stimulate IL-12 and IL-6 and to activate protease-activated receptors, PAR1 and PAR2 expressed on murine macrophages, was investigated. METHOD ANDRESULTS:
Using selected protease inhibitors, leupeptin and E64, we showed that Acanthamoeba proteases can stimulate IL-12 and IL-6 by murine macrophages. Subsequently, using specific antagonists to inhibit PAR1 , and bone marrow-derived macrophages from PAR2 gene-deficient mice, we demonstrate that PAR1 , but not PAR2 contributes to macrophage IL-12 production in response to Acanthamoeba. In contrast, Acanthamoeba-induced IL-6 production is PAR1 and PAR2 independent.CONCLUSION:
This study shows for the first time the involvement of PARs, expressed on macrophages, in the response to Acanthamoeba trophozoites and might provide useful insight into Acanthamoeba infections and their future treatments.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acanthamoeba
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Ciclo Celular
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Receptor PAR-2
/
Amebíase
/
Ativação de Macrófagos
/
Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Parasite Immunol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Reino Unido