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Cytomegalovirus vectors expressing Plasmodium knowlesi antigens induce immune responses that delay parasitemia upon sporozoite challenge.
Hansen, Scott G; Womack, Jennie; Scholz, Isabel; Renner, Andrea; Edgel, Kimberly A; Xu, Guangwu; Ford, Julia C; Grey, Mikayla; St Laurent, Brandyce; Turner, John M; Planer, Shannon; Legasse, Al W; Richie, Thomas L; Aguiar, Joao C; Axthelm, Michael K; Villasante, Eileen D; Weiss, Walter; Edlefsen, Paul T; Picker, Louis J; Früh, Klaus.
Afiliação
  • Hansen SG; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • Womack J; Oregon Health & Science University, Oregon National Primate Research Center, Beaverton, OR, United States of America.
  • Scholz I; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • Renner A; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • Edgel KA; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States of America.
  • Xu G; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States of America.
  • Ford JC; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • Grey M; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • St Laurent B; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • Turner JM; National Institutes of Health, Laboratory of Malaria and Vector Research, Malaria Pathogenesis and Human Immunity Unit, Rockville, MD, United States of America.
  • Planer S; Oregon Health & Science University, Oregon National Primate Research Center, Beaverton, OR, United States of America.
  • Legasse AW; Oregon Health & Science University, Oregon National Primate Research Center, Beaverton, OR, United States of America.
  • Richie TL; Oregon Health & Science University, Oregon National Primate Research Center, Beaverton, OR, United States of America.
  • Aguiar JC; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States of America.
  • Axthelm MK; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States of America.
  • Villasante ED; Oregon Health & Science University, Vaccine & Gene Therapy Institute, Beaverton, OR, United States of America.
  • Weiss W; Oregon Health & Science University, Oregon National Primate Research Center, Beaverton, OR, United States of America.
  • Edlefsen PT; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States of America.
  • Picker LJ; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States of America.
  • Früh K; Statistical Center for HIV/AIDS Research and Prevention, Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States of America.
PLoS One ; 14(1): e0210252, 2019.
Article em En | MEDLINE | ID: mdl-30673723
ABSTRACT
The development of a sterilizing vaccine against malaria remains one of the highest priorities for global health research. While sporozoite vaccines targeting the pre-erythrocytic stage show great promise, it has not been possible to maintain efficacy long-term, likely due to an inability of these vaccines to maintain effector memory T cell responses in the liver. Vaccines based on human cytomegalovirus (HCMV) might overcome this limitation since vectors based on rhesus CMV (RhCMV), the homologous virus in rhesus macaques (RM), elicit and indefinitely maintain high frequency, non-exhausted effector memory T cells in extralymphoid tissues, including the liver. Moreover, RhCMV strain 68-1 elicits CD8+ T cells broadly recognizing unconventional epitopes exclusively restricted by MHC-II and MHC-E. To evaluate the potential of these unique immune responses to protect against malaria, we expressed four Plasmodium knowlesi (Pk) antigens (CSP, AMA1, SSP2/TRAP, MSP1c) in RhCMV 68-1 or in Rh189-deleted 68-1, which additionally elicits canonical MHC-Ia-restricted CD8+ T cells. Upon inoculation of RM with either of these Pk Ag expressing RhCMV vaccines, we obtained T cell responses to each of the four Pk antigens. Upon challenge with Pk sporozoites we observed a delayed appearance of blood stage parasites in vaccinated RM consistent with a 75-80% reduction of parasite release from the liver. Moreover, the Rh189-deleted RhCMV/Pk vectors elicited sterile protection in one RM. Once in the blood, parasite growth was not affected. In contrast to T cell responses induced by Pk infection, RhCMV vectors maintained sustained T cell responses to all four malaria antigens in the liver post-challenge. The delayed appearance of blood stage parasites is thus likely due to a T cell-mediated inhibition of liver stage parasite development. As such, this vaccine approach can be used to efficiently test new T cell antigens, improve current vaccines targeting the liver stage and complement vaccines targeting erythrocytic antigens.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmodium knowlesi / Vacinas Antimaláricas / Parasitemia / Citomegalovirus / Esporozoítos / Malária / Antígenos de Protozoários Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmodium knowlesi / Vacinas Antimaláricas / Parasitemia / Citomegalovirus / Esporozoítos / Malária / Antígenos de Protozoários Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos