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Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells.
Gerli, Mattia Francesco Maria; Moyle, Louise Anne; Benedetti, Sara; Ferrari, Giulia; Ucuncu, Ekin; Ragazzi, Martina; Constantinou, Chrystalla; Louca, Irene; Sakai, Hiroshi; Ala, Pierpaolo; De Coppi, Paolo; Tajbakhsh, Shahragim; Cossu, Giulio; Tedesco, Francesco Saverio.
Afiliação
  • Gerli MFM; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK; Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, WC1N 1EH London, UK.
  • Moyle LA; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK.
  • Benedetti S; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK; Molecular and Cellular Immunology Section, Great Ormond Street Institute of Child Health, University College London, WC1N 1EH London, UK; NIHR Great Ormond Street Hospital Biomedical Research Centre, WC1N 1
  • Ferrari G; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK.
  • Ucuncu E; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK.
  • Ragazzi M; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK.
  • Constantinou C; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK.
  • Louca I; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK.
  • Sakai H; Department of Developmental & Stem Cell Biology, Institut Pasteur, 75015 Paris, France; CNRS UMR 3738, Institut Pasteur, 75015 Paris, France.
  • Ala P; The Dubowitz Neuromuscular Centre, Great Ormond Street Institute of Child Health, University College London, WC1N 1EH London, UK.
  • De Coppi P; Stem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College London, WC1N 1EH London, UK.
  • Tajbakhsh S; Department of Developmental & Stem Cell Biology, Institut Pasteur, 75015 Paris, France; CNRS UMR 3738, Institut Pasteur, 75015 Paris, France.
  • Cossu G; Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester, M13 9PL Manchester, UK.
  • Tedesco FS; Department of Cell and Developmental Biology, University College London, WC1E 6DE London, UK; The Dubowitz Neuromuscular Centre, Great Ormond Street Institute of Child Health, University College London, WC1N 1EH London, UK. Electronic address: f.s.tedesco@ucl.ac.uk.
Stem Cell Reports ; 12(3): 461-473, 2019 03 05.
Article em En | MEDLINE | ID: mdl-30745033
Satellite cells are responsible for skeletal muscle regeneration. Upon activation, they proliferate as transient amplifying myoblasts, most of which fuse into regenerating myofibers. Despite their remarkable differentiation potential, these cells have limited migration capacity, which curtails clinical use for widespread forms of muscular dystrophy. Conversely, skeletal muscle perivascular cells have less myogenic potential but better migration capacity than satellite cells. Here we show that modulation of Notch and PDGF pathways, involved in developmental specification of pericytes, induces perivascular cell features in adult mouse and human satellite cell-derived myoblasts. DLL4 and PDGF-BB-treated cells express markers of perivascular cells and associate with endothelial networks while also upregulating markers of satellite cell self-renewal. Moreover, treated cells acquire trans-endothelial migration ability while remaining capable of engrafting skeletal muscle upon intramuscular transplantation. These results extend our understanding of muscle stem cell fate plasticity and provide a druggable pathway with clinical relevance for muscle cell therapy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Biomarcadores / Transdução de Sinais / Movimento Celular / Receptores do Fator de Crescimento Derivado de Plaquetas / Células Satélites de Músculo Esquelético / Receptores Notch Limite: Animals / Humans Idioma: En Revista: Stem Cell Reports Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Biomarcadores / Transdução de Sinais / Movimento Celular / Receptores do Fator de Crescimento Derivado de Plaquetas / Células Satélites de Músculo Esquelético / Receptores Notch Limite: Animals / Humans Idioma: En Revista: Stem Cell Reports Ano de publicação: 2019 Tipo de documento: Article