Shedding light: The importance of reverse transcription efficiency standards in data interpretation.
Biomol Detect Quantif
; 17: 100077, 2019 Mar.
Article
em En
| MEDLINE
| ID: mdl-30805297
ABSTRACT
The RNA-to-cDNA conversion step in transcriptomics experiments is widely recognised as inefficient and variable, casting doubt on the ability to do quantitative transcriptomics analyses. Multiple studies have focused on ways to optimise this process, resulting in contradictory recommendations. Here we explore the problem of reverse transcription efficiency using digital PCR and the RT method's impact on subsequent data analysis. Using synthetic RNA standards, an example experiment is presented, outlining a method to (1) determine relevant efficiency and variability values and then to (2) incorporate this information into downstream analyses as a way to improve the accuracy of quantitative transcriptomics experiments.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biomol Detect Quantif
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Canadá