Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador.
Int J Tuberc Lung Dis
; 23(2): 166-173, 2019 02 01.
Article
em En
| MEDLINE
| ID: mdl-30808448
ABSTRACT
BACKGROUND:
The genetic diversity of Mycobacterium tuberculosis in Quito, Ecuador is not well known.OBJECTIVE:
To investigate mutations related to drug resistance and bacterial genotypes in M. tuberculosis strains in Ecuador.DESIGN:
This was a retrospective study of M. tuberculosis isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variable-number tandem repeats (MIRU-VNTR).RESULTS:
Isolates showed mutations in the rpoB and katG genes, and the inhA promoter. In rpoB, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In katG, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in katG Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed.CONCLUSION:
This is the first report from Ecuador; it describes five new mutations in katG and indicates that LAM is the most prevalent lineage.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Tuberculose Resistente a Múltiplos Medicamentos
/
Mycobacterium tuberculosis
/
Antituberculosos
Tipo de estudo:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
País/Região como assunto:
America do sul
/
Ecuador
Idioma:
En
Revista:
Int J Tuberc Lung Dis
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Equador