MiR-125b regulates the proliferation and metastasis of triple negative breast cancer cells via the Wnt/ß-catenin pathway and EMT.
Biosci Biotechnol Biochem
; 83(6): 1062-1071, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30950326
ABSTRACT
BACKGROUND/AIM:
MiR-125b plays an important role in breast cancer. The current study was to explore the expression and function of miR-125b in triple negative breast cancer cells. MATERIALS ANDMETHODS:
The expression of miR-125b in human TNBC samples and cell lines were examined by qRT-PCR. MTT, scratch assays and transwell assays were utilized to observe the proliferation, migration and invasion ability. MiR-125b's target gene and downstream signaling pathways were investigated by Luciferase Reporter Assays, qRT-PCR, immunofluorescence assays and western bolt.RESULTS:
MiR-125b was highly expressed in human TNBC tissues and cell lines. Inhibiting miR-125b expression suppressed the proliferation, cell migration and invasion. The three-prime untranslated region (3´-UTR) of adenomatous polyposis coli (APC) mRNA contains miR-125b binding sites, and inhibiting miR-125b expression suppressed the activity of the intracellular Wnt/ß-catenin pathways and EMT.CONCLUSION:
Inhibiting miR-125b regulates the Wnt/ß-catenin pathway and EMT to suppress the proliferation and migration of MDA-MB-468 TNBC cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
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Proliferação de Células
/
Beta Catenina
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Transição Epitelial-Mesenquimal
/
Via de Sinalização Wnt
/
Neoplasias de Mama Triplo Negativas
/
Metástase Neoplásica
Limite:
Female
/
Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China