Formation of lipoxin A by granulocytes from eosinophilic donors.
FEBS Lett
; 217(2): 242-6, 1987 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-3109942
ABSTRACT
The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(S), 6(R),15(S)-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Hidroxieicosatetraenoicos
/
Lipoxinas
/
Eosinofilia
/
Granulócitos
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1987
Tipo de documento:
Article