Hydroperoxides produced by n-6 lipoxygenation of arachidonic and linoleic acids potentiate synthesis of prostacyclin related compounds.
Biochim Biophys Acta
; 958(3): 460-8, 1988 Feb 19.
Article
em En
| MEDLINE
| ID: mdl-3124885
ABSTRACT
In a previous paper we reported that arachidonic acid (204(n-6] strongly enhances the endothelial cell synthesis of prostaglandin I3 (PGI3) from eicosapentaenoic acid (205(n-3], in stimulating the cyclooxygenase rather than the prostacyclin synthase (Bordet et al. (1986) Biochem. Biophys. Res. Commun. 135, 403-410). In the present study, endothelial cell monolayers were co-incubated with exogenous 205(n-3) or docosatetraenoic acid (224(n-6], and n-6 lipoxygenase products of 204(n-6) or linoleic acid (182(n-6], namely 15-HPETE and 13-HPOD, respectively. Prostaglandins or dihomoprostaglandins were then measured by gas chromatography-mass spectrometry. Both hydroperoxides, up to 20 microM, stimulated the cyclooxygenation of 205(n-3) and 224(n-6), in particular the formation of PGI3 and dihomo-PGI2, respectively. Higher concentrations inhibited prostacyclin synthetase. In contrast, the reduced products of hydroperoxides, 15-HETE and 13-HOD, failed to stimulate these cyclooxygenations, 13-HPOD appeared more potent than 15-HPETE and the cyclooxygenation of 224(n-6) seemed to require higher amounts of hydroperoxides to be efficiently metabolized than 205(n-3). These data suggest that prostacyclin potential of endothelium might be enhanced by raising the peroxide tone.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
/
Ácidos Linoleicos
/
Ácidos Araquidônicos
/
Leucotrienos
/
Epoprostenol
/
Lipoxigenase
/
Peróxidos Lipídicos
Limite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
França