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The Role of a Host-Induced Arginase of Xanthomonas oryzae pv. oryzae in Promoting Virulence on Rice.
Zhang, Yuqiang; Wu, Guichun; Palmer, Ian; Wang, Bo; Qian, Guoliang; Fu, Zheng Qing; Liu, Fengquan.
Afiliação
  • Zhang Y; Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, P.R. China.
  • Wu G; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, P.R. China.
  • Palmer I; Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, U.S.A.
  • Wang B; Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, P.R. China.
  • Qian G; Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, P.R. China.
  • Fu ZQ; Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, U.S.A.
  • Liu F; Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, P.R. China.
Phytopathology ; 109(11): 1869-1877, 2019 Nov.
Article em En | MEDLINE | ID: mdl-31290730
The plant bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial blight of rice, which is one of the most destructive rice diseases prevalent in Asia and parts of Africa. Despite many years of research, how X. oryzae pv. oryzae causes bacterial blight of rice is still not completely understood. Here, we show that the loss of the rocF gene caused a significant decrease in the virulence of X. oryzae pv. oryzae in the susceptible rice cultivar IR24. Bioinformatics analysis demonstrated that rocF encodes arginase. Quantitative real-time PCR and Western blot assays revealed that rocF expression was significantly induced by rice and arginine. The rocF deletion mutant strain showed elevated sensitivity to hydrogen peroxide, reduced extracellular polysaccharide (EPS) production, and reduced biofilm formation, all of which are important determinants for the full virulence of X. oryzae pv. oryzae, compared with the wild-type strain. Taken together, the results of this study revealed a mechanism by which a bacterial arginase is required for the full virulence of X. oryzae pv. oryzae on rice because of its contribution to tolerance to reactive oxygen species, EPS production, and biofilm formation.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arginase / Oryza / Xanthomonas / Regulação Bacteriana da Expressão Gênica / Interações Hospedeiro-Patógeno País/Região como assunto: Africa / Asia Idioma: En Revista: Phytopathology Assunto da revista: BOTANICA Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arginase / Oryza / Xanthomonas / Regulação Bacteriana da Expressão Gênica / Interações Hospedeiro-Patógeno País/Região como assunto: Africa / Asia Idioma: En Revista: Phytopathology Assunto da revista: BOTANICA Ano de publicação: 2019 Tipo de documento: Article