Quantitation of phosphatidylserine-exposing platelets in platelet concentrate prepared in routine blood transfusion laboratory.
Transfus Apher Sci
; 59(1): 102598, 2020 Feb.
Article
em En
| MEDLINE
| ID: mdl-31303511
ABSTRACT
BACKGROUND:
Phosphatidylserine (PS) plays important roles in platelets' pro-coagulant function. However, little is known about assessing this molecule in platelet concentrates (PCs) prepared for routine blood transfusion service.AIM:
To quantitate the number of PS-exposing platelets in PCs prepared in a routine transfusion laboratory.METHODS:
PC products were prepared according to routine laboratory procedure. The numbers of PS-exposing platelets in the PCs and in unprocessed whole blood were determined using flow cytometry.RESULTS:
A cross-sectional study of 253 PCs found that they had significantly increased numbers of PS-exposing platelets compared to unprocessed whole blood (47,439⯱â¯26,500â¯cells/µL; 5903â166,156â¯cells/µL) vs. 30,058⯱â¯12,958â¯cells/µL; 8,154-86,606â¯cells/µL). A heterogeneity study demonstrated that 6% and 2% of the measured PCs and of unprocessed donor whole blood, respectively, showed an increase in the number of PS-exposing platelets that was greater than 2 fold.CONCLUSIONS:
The study suggested that the number PS-exposing platelets in PC prepared in a routine transfusion laboratory differs. However, assessment of the number of PS-exposing platelets in platelet products could be a valid measure to use in managing the quality of platelet processing in routine laboratories.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilserinas
/
Plaquetas
/
Transfusão de Sangue
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Transfus Apher Sci
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article