Chemical cross-linking and its effect on fatty acid synthetase activity in intact chloroplasts from Euglena gracilis.
Biochim Biophys Acta
; 963(3): 423-8, 1988 Dec 16.
Article
em En
| MEDLINE
| ID: mdl-3143414
ABSTRACT
Intact chloroplasts were isolated from Euglena gracilis variety bacillaris, aliquots were exposed to several different chemical cross-linking reagents. The reagents penetrated the triple membrane of Euglena chloroplasts. This was shown by gradient acrylamide gel electrophoresis under denaturing conditions. The activity of the nonaggregated fatty acid synthetase of Euglena was located within the chloroplast stroma, and the effects of dimethylsuberimidate cross-linking on the activity of the enzyme system were examined. The acyl-carrier protein concentration in the chloroplast was measured at about 0.24 mM.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cloroplastos
/
Reagentes de Ligações Cruzadas
/
Euglena
/
Ácido Graxo Sintases
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1988
Tipo de documento:
Article