Characterization of Withania somnifera chloroplast genome and its comparison with other selected species of Solanaceae.
Genomics
; 112(2): 1522-1530, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31470082
ABSTRACT
Withania somnifera (L) Dunal, a wonder herb of family Solanaceae, has multiple medicinal properties. Here, we reported the chloroplast genome sequence of Withania somnifera (154,386â¯bp) which comprises of a large single copy region (85,688â¯bp), and a small single copy region (18,464â¯bp), separated by a pair of large inverted repeats (25,117â¯bp). The chloroplast genome has 132 genes including 86 protein-coding, 37 tRNAs and 8 rRNAs. Comparison of chloroplast genomes of Withania somnifera with four other Solanaceae species revealed similarities in genomic features, including structure, nucleotide content, codon usage, RNA editing sites, simple sequence repeats (SSRs), oligonucleotide repeats, and tandem repeats. We identified 147 simple sequence repeats in protein-coding, and 229 in non-protein-coding regions. We observed numerous post-transcriptional substitutions of Serine to Leucine, specifically at the second nucleotide position of the codon. Maximum likelihood and maximum parsimony tree reconstructed displayed Withania somnifera a sister taxon of Physalis peruviana.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Withania
/
Genoma de Cloroplastos
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Paquistão