Liquid chromatography and gas chromatography/mass spectrometry of lipoxygenase and cyclooxygenase products from platelets and endothelial cells.

ABSTRACT

Liquid chromatography coupled with mass spectrometry using either positive or negative ionization was used for measuring various lipoxygenase products of polyunsaturated fatty acids. The negative ionization appeared as the most sensitive mode and allowed to detect pmol amounts of products from biological extracts. Gas chromatography/mass spectrometry with the negative chemical ionization mode was also used for measuring prostacyclin synthetase products, namely the stable metabolites of PGI2, PGI3 and dihomo PGI2. In this way, fmol amounts of metabolites could be measured in biological extracts.