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Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.
Zawieja, David C; Thangaswamy, Sangeetha; Wang, Wei; Furtado, Raquel; Clement, Cristina C; Papadopoulos, Zachary; Vigano, Marco; Bridenbaugh, Eric A; Zolla, Lello; Gashev, Anatoliy A; Kipnis, Jonathan; Lauvau, Gregoire; Santambrogio, Laura.
Afiliação
  • Zawieja DC; Department of Medical Physiology, Texas A&M Health Science Center, Temple, TX 76504.
  • Thangaswamy S; Department of Pathology, Albert Einstein College of Medicine, Montefiore Medical Center, New York, NY 10461.
  • Wang W; Department of Medical Physiology, Texas A&M Health Science Center, Temple, TX 76504.
  • Furtado R; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Montefiore Medical Center, New York, NY 10461.
  • Clement CC; Department of Pathology, Albert Einstein College of Medicine, Montefiore Medical Center, New York, NY 10461.
  • Papadopoulos Z; Center for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, VA 22908.
  • Vigano M; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22908.
  • Bridenbaugh EA; Department of Pathology, Albert Einstein College of Medicine, Montefiore Medical Center, New York, NY 10461.
  • Zolla L; Orthopaedic Biotechnology Lab, Galeazzi Orthopaedic Institute for Care and Scientific Research, 20161 Milan, Italy; and.
  • Gashev AA; Department of Medical Physiology, Texas A&M Health Science Center, Temple, TX 76504.
  • Kipnis J; Orthopaedic Biotechnology Lab, Galeazzi Orthopaedic Institute for Care and Scientific Research, 20161 Milan, Italy; and.
  • Lauvau G; Department of Medical Physiology, Texas A&M Health Science Center, Temple, TX 76504.
  • Santambrogio L; Center for Brain Immunology and Glia, School of Medicine, University of Virginia, Charlottesville, VA 22908.
J Immunol ; 203(8): 2339-2350, 2019 10 15.
Article em En | MEDLINE | ID: mdl-31519866
Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cateterismo / Vasos Linfáticos / Linfa / Linfonodos Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cateterismo / Vasos Linfáticos / Linfa / Linfonodos Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 2019 Tipo de documento: Article