Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.
J Immunol
; 203(8): 2339-2350, 2019 10 15.
Article
em En
| MEDLINE
| ID: mdl-31519866
Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cateterismo
/
Vasos Linfáticos
/
Linfa
/
Linfonodos
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
2019
Tipo de documento:
Article