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ORANGE Represses Chloroplast Biogenesis in Etiolated Arabidopsis Cotyledons via Interaction with TCP14.
Sun, Tianhu; Zhou, Fei; Huang, Xing-Qi; Chen, Wei-Cai; Kong, Meng-Juan; Zhou, Chang-Fang; Zhuang, Zhong; Li, Li; Lu, Shan.
Afiliação
  • Sun T; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Zhou F; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service, and Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853.
  • Huang XQ; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Chen WC; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Kong MJ; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Zhou CF; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Zhuang Z; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Li L; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Lu S; Robert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service, and Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853.
Plant Cell ; 31(12): 2996-3014, 2019 12.
Article em En | MEDLINE | ID: mdl-31604812
ABSTRACT
The conversion of etioplasts into chloroplasts in germinating cotyledons is a crucial transition for higher plants, enabling photoautotrophic growth upon illumination. Tight coordination of chlorophyll biosynthesis and photosynthetic complex assembly is critical for this process. ORANGE (OR), a DnaJ-like zinc finger domain-containing protein, was reported to trigger the biogenesis of carotenoid-accumulating plastids by promoting carotenoid biosynthesis and sequestration. Both nuclear and plastidic localizations of OR have been observed. Here, we show that Arabidopsis (Arabidopsis thaliana) OR physically interacts with the transcription factor TCP14 in the nucleus and represses its transactivation activity. Through this interaction, the nucleus-localized OR negatively regulates expression of EARLY LIGHT-INDUCIBLE PROTEINS (ELIPs), reduces chlorophyll biosynthesis, and delays development of thylakoid membranes in the plastids of germinating cotyledons. Nuclear abundance of OR decreased upon illumination. Together with an accumulation of TCP14 in the nucleus, this derepresses chloroplast biogenesis during de-etiolation. TCP14 is epistatic to OR and expression of ELIPs is directly regulated by the binding of TCP14 to Up1 elements in the ELIP promoter regions. Our results demonstrate that the interaction between OR and TCP14 in the nucleus leads to repression of chloroplast biogenesis in etiolated seedlings and provide new insights into the regulation of early chloroplast development.plantcell;31/12/2996/FX1F1fx1.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Clorofila / Cloroplastos / Arabidopsis / Cotilédone / Proteínas de Arabidopsis / Proteínas de Choque Térmico HSP40 Idioma: En Revista: Plant Cell Assunto da revista: BOTANICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Clorofila / Cloroplastos / Arabidopsis / Cotilédone / Proteínas de Arabidopsis / Proteínas de Choque Térmico HSP40 Idioma: En Revista: Plant Cell Assunto da revista: BOTANICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China